Some of the chemicals used in the procedures—acrylamide,
N,N’-methylenebisacrylamide, ammonium persulfate, TEMED, DTT,
iodoacetamide, and DeStreak Reagent—are very hazardous. Acrylamide
monomer, for example, is a neurotoxin and suspected carcinogen.
Read the manufacturer’s safety data sheet (MSDS) detailing the
properties and precautions for all chemicals in your laboratory.
These safety data sheets should be reviewed prior to starting the
procedures described in this handbook. General handling procedures
for hazardous chemicals include using double latex gloves for all
protocols. Hazardous materials should be weighed in a fume hood
while wearing a disposable dust mask. Follow all local rules and
regulations for handling and disposal of materials.